Anti-Human CD1a Antibody (2113) (HY043107)

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Overview

Catalog No.	HY043107
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	2113
Clonality	Monoclonal
Target	T-cell surface glycoprotein CD1a, CD1A, T-cell surface antigen T6/Leu-6, CD1a, hTa1 thymocyte antigen
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P06126
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Human CD1a Antibody (2113).
Flow Cytometry

Flow-cytometry using anti-human CD1a antibody. Human MOLT-4 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD1a antibody monoclonal antibody (Catalog # HY043107 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD1a antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD1a antibody monoclonal antibody (Catalog # HY043107 , Red Histogram) at a concentration of 20µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet