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Anti-Human CD246/ALK Antibody (ab324) (HV427107)

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Anti-Human CD246/ALK Antibody (ab324)

Anti-Human CD246/ALK Antibody (ab324)

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Datasheet

Overview

Catalog No.	HV427107
Description	Anti-Human CD246/ALK Antibody (ab324) [ab324] (HV427107) is a mouse monoclonal antibody detecting CD246 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	ab324
Clonality	Monoclonal
Target	ALK, ALK tyrosine kinase receptor, CD246, Anaplastic lymphoma kinase
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9UM73
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	ALK tyrosine kinase receptor (CD246) is a ~176 kDa protein. Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Also acts as a key thinness protein involved in the resistance to weight gain: in hypothalamic neurons, controls energy expenditure acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis. Following activation by ALKAL2 ligand at the cell surface, transduces an extracellular signal into an intracellular response. In contrast, ALKAL1 is not a potent physiological ligand for ALK. Ligand-binding to the extracellular domain induces tyrosine kinase activation, leading to activation of the mitogen-activated protein kinase (MAPK) pathway. 1. Souttou, B. et al. (2001) The Journal of biological chemistry 276, 9526-31. PMID: 11121404 2. Simonitsch, I. et al. (2001) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 15, 1416-8. PMID: 11387242 3. Gouzi, JY. et al. (2005) Journal of cell science 118, 5811-23. PMID: 16317043 4. Degoutin, J. et al. (2007) FEBS letters 581, 727-34. PMID: 17274988 5. Reshetnyak, AV. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 8340-8345. PMID: 30061385 6. De Munck, S. et al. (2021) Nature 600, 143-147. PMID: 34646012 7. Reshetnyak, AV. et al. (2021) Nature 600, 153-157. PMID: 34819673 8. Borenäs, M. et al. (2021) The EMBO journal 40, e105784. PMID: 33411331
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CD246 antibody.CD246 Transfected HI5 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD246 antibody monoclonal antibody (Catalog # HV427107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD246/ALK Antibody (ab324).pdf

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Anti-Human CD246/ALK Antibody (ab324)