Anti-Human CD270/TNFRSF14 Antibody (36H12) (HV480107)

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Overview

Catalog No.	HV480107
Description	Anti-Human CD270/TNFRSF14 Antibody (36H12) [36H12] (HV480107) is a mouse monoclonal antibody detecting TNFRSF14 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	36H12
Clonality	Monoclonal
Target	HVEA, HVEM, Herpes virus entry mediator A, Tumor necrosis factor receptor superfamily member 14, Tumor necrosis factor receptor-like 2, HveA, TNFRSF14, CD270, TR2, Herpesvirus entry mediator A
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q92956
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Tumor necrosis factor receptor superfamily member 14 (CD270/TNFRSF14) is a ~30 kDa protein. Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network. Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation. Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions. Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response. 1. Tamada, K. et al. (2000) Journal of immunology (Baltimore, Md. : 1950) 164, 4105-10. PMID: 10754304 2. Cai, G. et al. (2008) Nature immunology 9, 176-85. PMID: 18193050 3. Šedý, JR. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 828-36. PMID: 23761635 4. Mauri, DN. et al. (1998) Immunity 8, 21-30. PMID: 9462508 5. Cheung, TC. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 183, 7286-96. PMID: 19915044 6. Hsu, H. et al. (1997) The Journal of biological chemistry 272, 13471-4. PMID: 9153189 7. Marsters, SA. et al. (1997) The Journal of biological chemistry 272, 14029-32. PMID: 9162022
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CD270 antibody.CD270 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD270 antibody monoclonal antibody (Catalog # HV480107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

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Datasheet

Anti-Human CD270/TNFRSF14 Antibody (36H12).pdf

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Anti-Human CD270/TNFRSF14 Antibody (36H12) (HV480107)

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