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Anti-Human CD32/FCGR2A Antibody (IV.3) (HY139107)

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Anti-Human CD32/FCGR2A Antibody (IV.3)

Anti-Human CD32/FCGR2A Antibody (IV.3)

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Datasheet

Overview

Catalog No.	HY139107
Description	Anti-Human CD32/FCGR2A Antibody (IV.3) [IV.3] (HY139107) is a human monoclonal antibody detecting FCGR2A in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	IV.3
Clonality	Monoclonal
Target	Fc-gamma RII-a, IgG Fc receptor II-a, IGFR2, FCG2, CDw32, FCGR2A1, FCGR2A, FcRII-a, CD32, Low affinity immunoglobulin gamma Fc region receptor II-a, Fc-gamma-RIIa
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P12318
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Low affinity immunoglobulin gamma Fc region receptor II-a (CD32/FCGR2A) is a ~35 kDa protein. Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CD32 antibody.CD32 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD32 antibody monoclonal antibody (Catalog # HY139107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD32/FCGR2A Antibody (IV.3).pdf

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Anti-Human CD32/FCGR2A Antibody (IV.3)