









| Catalog No. | HY286207 |
|---|---|
| Species reactivity | Human |
| Applications | FCM |
| Host species | Mouse |
| Isotype | IgG2a, kappa |
| Clone ID | MAB 2C4 |
| Clonality | Monoclonal |
| Target | p185erbB2, HER2, NGL, Tyrosine kinase-type cell surface receptor HER2, NEU, MLN 19, Proto-oncogene Neu, MLN19, ERBB2, Proto-oncogene c-ErbB-2, CD340, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P04626 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |

Flow-cytometry using anti-human CD340 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 antibody monoclonal antibody (Catalog # HY286207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD340 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 antibody monoclonal antibody (Catalog # HY286207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD340 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 antibody monoclonal antibody (Catalog # HY286207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD340 antibody.SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 antibody monoclonal antibody (Catalog # HY286207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SEC-HPLC detection for Anti-Human CD340/ERBB2/HER2 Antibody (MAB 2C4).
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