Anti-Human CD355/CRTAM Antibody (5A11) (HF645107)

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Overview

Catalog No.	HF645107
Description	Anti-Human CD355/CRTAM Antibody (5A11) [5A11] (HF645107) is a mouse monoclonal antibody detecting CD355 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	5A11
Clonality	Monoclonal
Target	Class-I MHC-restricted T-cell-associated molecule, Cytotoxic and regulatory T-cell molecule, CD355, CRTAM
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	O95727
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Cytotoxic and regulatory T-cell molecule (CD355/CRTAM) is a ~44 kDa protein. Mediates heterophilic cell-cell adhesion which regulates the activation, differentiation and tissue retention of various T-cell subsets. Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and IFNG/interferon-gamma secretion by CD8+ T-cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo. Regulates CD8+ T-cell proliferation in response to T-cell receptor (TCR) activation. Appears to be dispensable for CD8+ T-cell-mediated cytotoxicity. Interaction with SCRIB promotes the late phase of cellular polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG, IL17 and IL22 production. 1. Boles, KS. et al. (2005) Blood 106, 779-86. PMID: 15811952
Note	For research use only.

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Bioactivity

Detects CD355/CRTAM in indirect ELISAs.
Western blot

Western blot analysis was performed using anti-CD355/CRTAM monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD355/CRTAM (Catalog No: HF645011)
Lane 2: negative control

References

Datasheet

Anti-Human CD355/CRTAM Antibody (5A11).pdf

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Anti-Human CD355/CRTAM Antibody (5A11) (HF645107)

Overview

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Datasheet

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