





| Catalog No. | HY477107 | 
|---|---|
| Species reactivity | Human | 
| Applications | FCM, WB | 
| Host species | Mouse | 
| Isotype | IgG1, kappa | 
| Clone ID | 5G6 | 
| Clonality | Monoclonal | 
| Target | CD42b, GP-Ib alpha, Antigen CD42b-alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Platelet glycoprotein Ib alpha chain, GP1BA | 
| Endotoxin level | Please contact with the lab for this information. | 
| Purity | >95% as determined by SDS-PAGE. | 
| Purification | Protein A/G purified from cell culture supernatant. | 
| Accession | P07359 | 
| Form | Liquid | 
| Storage buffer | 0.01M PBS, pH 7.4.                                                 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.  | 
                                        
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | 
| Note | For research use only. | 

Detects CD42b/GP1BA in indirect ELISAs.

Flow-cytometry using anti-human CD42 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD42 antibody monoclonal antibody (Catalog # HY477107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Western blot analysis was performed using anti-CD42b/GP1BA  monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD42b/GP1BA  (Catalog No: HY477011)
Lane 2: negative control




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