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Anti-Human CD47/MER6 Antibody (B6H12) (HC359207)

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Overview
Catalog No.HC359207
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG1, kappa
Clone IDB6H12
ClonalityMonoclonal
TargetLeukocyte surface antigen CD47, CD47, Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Protein MER6, MER6
Endotoxin levelPlease contact with the lab for this information.
Purity>95% as determined by SDS-PAGE.
PurificationProtein A/G purified from cell culture supernatant.
AccessionQ08722
FormLiquid
Storage buffer0.01M PBS, pH 7.4.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
NoteFor research use only.
Images

  • SEC-HPLC

    SEC-HPLC detection for Anti-Human CD47/MER6 Antibody (B6H12).

  • Bioactivity

    Detects CD47/MER6 in indirect ELISAs.

  • Flow Cytometry

    Flow-cytometry using anti-human CD47 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD47 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD47 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD47 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # HC359207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

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Anti-Human CD47/MER6 Antibody (B6H12)

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