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Anti-Human CD5 Antibody (5D7) (HY046207)

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Anti-Human CD5 Antibody (5D7)

Anti-Human CD5 Antibody (5D7)

Anti-Human CD5 Antibody (5D7)

Anti-Human CD5 Antibody (5D7)

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Datasheet

Overview

Catalog No.	HY046207
Description	Anti-Human CD5 Antibody (5D7) [5D7] (HY046207) is a mouse monoclonal antibody detecting CD5 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1
Clone ID	5D7
Clonality	Monoclonal
Target	Lymphocyte antigen T1/Leu-1, CD5, T-cell surface glycoprotein CD5, LEU1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P06127
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	T-cell surface glycoprotein CD5 is a ~54 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. 1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049 2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158 3. Roa, NS. et al. (2013) Biochemical and biophysical research communications 432, 52-9. PMID: 23376399 4. Garaud, S. et al. (2018) Cellular & molecular immunology 15, 158-170. PMID: 27499044
Note	For research use only.

Images

Bioactivity

Detects CD5 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Human CD5 Antibody (5D7)

References

Datasheet

Anti-Human CD5 Antibody (5D7).pdf

Formula

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Anti-Human CD5 Antibody (5D7)