Anti-Human CD52 Recombinant Antibody (SAA0024) (HB894207)

Name: Anti-Human CD52 Recombinant Antibody (SAA0024)
Brand: abinScience
SKU: HB894207
Price: 196 USD
Availability: InStock

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Overview

Catalog No.	HB894207
Species reactivity	Human
Applications	FCM, WB
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	SAA0024
Clonality	Monoclonal
Target	Human epididymis-specific protein 5, CDW52, CAMPATH-1 antigen, Cambridge pathology 1 antigen, He5, HE5, Epididymal secretory protein E5, CD52, CDw52
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P31358
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	CAMPATH-1 antigen (CD52) is a ~6 kDa protein. May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human CD52 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD52 antibody monoclonal antibody (Catalog # HB894207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD52 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD52 monoclonal antibody (Catalog: HB894207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD52 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD52 (Catalog No: HB894011)
Lane 2: negative control