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Anti-HBV HBsAg/preS1 Recombinant Antibody (G10), PE (VK539327)

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Overview
Catalog No.VK539327
Description
Anti-HBV HgsAg/preS1 Recombinant Antibody (G10), PE (VK539327) is a human monoclonal antibody detecting HBV HBsAg,preS1 in FCM. Suitable for Hepatitis B virus (HBV).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivityHepatitis B virus (HBV)
ApplicationsFCM
Host speciesHuman
ClonalityMonoclonal
IsotypeIgG1
Clone IDG10
ConjugationPE
Target Large envelope protein S, L glycoprotein, L-HBsAg, LHB, Large surface protein, Major surface protein, preS1 domain, Pre-S1
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P03141
Form Liquid
Storage buffer 0.01M PBS pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Large envelope protein (HBsAg) is a ~42 kDa protein. The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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