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Anti-Mouse CD3E Antibody (500A2) (MY057307)

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Anti-Mouse CD3E Antibody (500A2)

Anti-Mouse CD3E Antibody (500A2)

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Overview

Catalog No.	MY057307
Description	Anti-Mouse CD3E Antibody (500A2) [500A2] (MY057307) is a hamster monoclonal antibody detecting CD3e in FCM. Suitable for Mouse. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Mouse
Applications	FCM
Host species	Hamster
Isotype	IgG1
Clone ID	500A2
Clonality	Monoclonal
Target	T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P22646
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~21 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto). 1. DeJarnette, JB. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 14909-14. PMID: 9843989 2. Saint-Ruf, C. et al. (2000) Nature 406, 524-7. PMID: 10952314 3. Wang, Y. et al. (2009) PLoS biology 7, e1000253. PMID: 19956738 4. Borroto, A. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 2042-53. PMID: 24470497 5. Bettini, ML. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 258-67. PMID: 24899501 6. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325
Note	For research use only.

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SEC-HPLC detection for Anti-Mouse CD3E Antibody (500A2).

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Datasheet

Anti-Mouse CD3E Antibody (500A2).pdf

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Anti-Mouse CD3E Antibody (500A2)