Anti-Mouse PD-1 Recombinant Antibody (J43V2) (MS870107)

Name: Anti-Mouse PD-1 Recombinant Antibody (J43V2)
Brand: abinScience
SKU: MS870107
Price: 163 USD
Availability: InStock

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Overview

Catalog No.	MS870107
Description	Anti-Mouse PD-1 Recombinant Antibody (J43V2) (MS870107) is a mouse monoclonal antibody detecting PD-1 in FCM. Suitable for Mouse. Highlights ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivity	Mouse
Applications	FCM
Host species	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone ID	J43V2
Tested applications	FCM
Target	CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, PD-1
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q02242
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Programmed cell death protein 1 (CD279/PD-1) is a ~31 kDa protein. Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival. CD279 is the therapeutic target of pembrolizumab (Keytruda) and nivolumab (Opdivo). 1. Nishimura, H. et al. (1999) Immunity 11, 141-51. PMID: 10485649 2. Nishimura, H. et al. (2001) Science (New York, N.Y.) 291, 319-22. PMID: 11209085 3. Okazaki, T. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 13866-71. PMID: 11698646 4. Okazaki, T. et al. (2011) The Journal of experimental medicine 208, 395-407. PMID: 21300912 5. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 6. Latchman, Y. et al. (2001) Nature immunology 2, 261-8. PMID: 11224527 7. Lin, DY. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 3011-6. PMID: 18287011 8. Lázár-Molnár, E. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 10483-8. PMID: 18641123 9. Yokosuka, T. et al. (2012) The Journal of experimental medicine 209, 1201-17. PMID: 22641383
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Mouse CD279/PDCD1/PD1 Antibody (J43V2).
Flow Cytometry

Flow-cytometry using anti-mouse CD279 antibody.CD279 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD279 antibody monoclonal antibody (Catalog # MS870107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects recombinant mouse CD279/PDCD1/PD1 (Catalog No: MS870011) in indirect ELISA

References

Datasheet

Anti-Mouse PD-1 Recombinant Antibody (J43V2).pdf

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Formula

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Anti-Mouse PD-1 Recombinant Antibody (J43V2) (MS870107)

Overview

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References

Datasheet

SDS