Anti-Mouse CD79b Antibody (HM79-16) (MW431107)

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Overview

Catalog No.	MW431107
Description	Anti-Mouse CD79b Antibody (HM79-16) [HM79-16] (MW431107) is a mouse monoclonal antibody detecting CD79b in FCM. Suitable for Mouse. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Mouse
Applications	FCM
Host species	Mouse
Isotype	IgG2a, lambda
Clone ID	HM79-16
Clonality	Monoclonal
Target	B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig-beta, Immunoglobulin-associated B29 protein, CD79b, Igb
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P15530
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	B-cell antigen receptor complex-associated protein beta chain (CD79b) is a ~25 kDa protein. Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-mouse CD79b antibody.CD79b Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD79b antibody monoclonal antibody (Catalog # MW431107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

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Datasheet

Anti-Mouse CD79b Antibody (HM79-16).pdf

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Anti-Mouse CD79b Antibody (HM79-16) (MW431107)

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