Anti-Mouse CD154/CD40L/TNFSF5 Antibody (MR1/MR-1) (MB199107)

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Overview

Catalog No.	MB199107
Description	Anti-Mouse CD154/CD40L/TNFSF5 Antibody (MR1/MR-1) [MR1/MR-1] (MB199107) is a rat monoclonal antibody detecting Tnfsf5 in Blocking, FCM, IHC. Suitable for Mouse. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Mouse
Applications	Blocking, FCM, IHC
Host species	Rat
Isotype	IgG1, kappa
Clone ID	MR1/MR-1
Clonality	Monoclonal
Target	CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, Cd40lg, Cd40l, Tnfsf5
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P27548
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	CD40 ligand (CD154/CD40L) is a ~29 kDa protein. Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. 1. Armitage, RJ. et al. (1992) Nature 357, 80-2. PMID: 1374165
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-mouse CD154 antibody.CD154 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD154 antibody monoclonal antibody (Catalog # MB199107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.

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Datasheet

Anti-Mouse CD154/CD40L/TNFSF5 Antibody (MR1/MR-1).pdf

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Anti-Mouse CD154/CD40L/TNFSF5 Antibody (MR1/MR-1) (MB199107)

Overview

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Datasheet

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