Anti-Human IL-10 Recombinant Antibody (SAA0378) (HB997107)

Name: Anti-Human IL-10 Recombinant Antibody (SAA0378)
Brand: abinScience
SKU: HB997107
Price: 196 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.	HB997107
Species reactivity	Human
Applications	Blocking, ELISA, FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	SAA0378
Clonality	Monoclonal
Tested applications	FCM
Target	IL-10, IL10, Cytokine synthesis inhibitory factor, CSIF, Interleukin-10
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P22301
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Interleukin-10 (IL10) is a ~20 kDa protein. Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. 1. Yoon, SI. et al. (2006) The Journal of biological chemistry 281, 35088-96. PMID: 16982608 2. El Kasmi, KC. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 7215-9. PMID: 18025162 3. Hashimoto, SI. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 3619-25. PMID: 11564774 4. de Waal Malefyt, R. et al. (1991) The Journal of experimental medicine 174, 1209-20. PMID: 1940799 5. Willems, F. et al. (1994) European journal of immunology 24, 1007-9. PMID: 7512027 6. Frei, K. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 2720-8. PMID: 8144879
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human IL10 antibody.IL10 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL10 antibody monoclonal antibody (Catalog # HB997107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet