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Anti-Human GM-CSF Recombinant Antibody (SAA0418) (HY213107)

Anti-Human GM-CSF Recombinant Antibody (SAA0418)
Anti-Human GM-CSF Recombinant Antibody (SAA0418)
Anti-Human GM-CSF Recombinant Antibody (SAA0418)
Anti-Human GM-CSF Recombinant Antibody (SAA0418)
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Overview
Catalog No.HY213107
Species reactivityHuman
ApplicationsELISA, FCM
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA0418
Clonality Monoclonal
Target Granulocyte-macrophage colony-stimulating factor, Sargramostim, GMCSF, CSF, Colony-stimulating factor, CSF2, GM-CSF, Molgramostin
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P04141
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Granulocyte-macrophage colony-stimulating factor (CSF2/GM-CSF) is a ~16 kDa protein. Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Note For research use only.
Images
  • Anti-Human GM-CSF Recombinant Antibody (SAA0418)

    Flow-cytometry

    Flow-cytometry using anti-human CSF2 antibody.CSF2 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF2 antibody monoclonal antibody (Catalog # HY213107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human GM-CSF Recombinant Antibody (SAA0418)

    Flow-cytometry

    Flow-cytometry using anti-human CSF2 antibody.HL-60 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF2 antibody monoclonal antibody (Catalog # HY213107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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