Anti-Mouse CD45/PTPRC Antibody (SAA0570) (MY195107)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.	MY195107
Description	Anti-Mouse CD45/PTPRC Antibody (SAA0570) [SAA0570] (MY195107) is a mouse monoclonal antibody detecting PTPRC in FCM. Suitable for Mouse. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Mouse
Applications	FCM
Host species	Mouse
Isotype	VHH, mFc (IgG2a)
Clone ID	SAA0570
Clonality	Monoclonal
Target	T200, Leukocyte common antigen, CD45, L-CA, Receptor-type tyrosine-protein phosphatase C, PTPRC
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P06800
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Receptor-type tyrosine-protein phosphatase C (CD45/PTPRC) is a ~144 kDa protein. Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-mouse CD45 antibody.Raw264.7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD45 antibody monoclonal antibody (Catalog # MY195107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Mouse CD45/PTPRC Antibody (SAA0570).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD45/PTPRC Antibody (SAA0570) (MY195107)

Overview

Images

References

Datasheet

Quick access

Contact Us