Anti-Human GPC2/Glypican-2 Antibody (D3) (HP003207)

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Overview

Catalog No.	HP003207
Description	Anti-Human GPC2/Glypican-2 Antibody (D3) [D3] (HP003207) is a mouse monoclonal antibody detecting Glypican-2 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	D3
Clonality	Monoclonal
Target	Glypican-2, GPC2
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q8N158
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Glypican-2 (GPC2) is a ~62 kDa protein. Cell surface proteoglycan that bears heparan sulfate. May fulfill a function related to the motile behaviors of developing neurons.
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human GPC2/Glypican-2 Antibody (D3).
Flow Cytometry

Flow-cytometry using anti-human GPC2 antibody. Human GPC2 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC2 antibody monoclonal antibody (Catalog # HP003207 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Formula

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Working Solution

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Overview