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Anti-Mouse CD40/TNFRSF5 Antibody (FGK45), FITC (MB782117)

Anti-Mouse CD40/TNFRSF5 Antibody (FGK45), FITC
Anti-Mouse CD40/TNFRSF5 Antibody (FGK45), FITC
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  • 50T
  • 100T
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Overview
Catalog No.MB782117
Description
Anti-Mouse CD40/TNFRSF5 Antibody (FGK45), FITC [FGK45] (MB782117) is a rat monoclonal antibody detecting TNFRSF5 in FCM. Suitable for Mouse.
Highlights
  • Flow Cytometry — Validated for flow cytometry applications.
  • Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivityMouse
ApplicationsFCM
Host speciesRat
IsotypeIgG2a, kappa
Clone IDFGK45
ConjugationFITC
Clonality Monoclonal
Target Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, TNFRSF5, CDw40, CD40
Endotoxin level Please contact with the lab for this information.
Accession P27512
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Tumor necrosis factor receptor superfamily member 5 (CD40/TNFRSF5) is a ~32 kDa protein. Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

1. Eliopoulos, AG. et al. (2003) The EMBO journal 22, 3855-64. PMID: 12881420
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Mouse CD40/TNFRSF5 Antibody (FGK45), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-Mouse CD40 antibody. Mouse spleen cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-Mouse CD40 monoclonal antibody (Catalog: MB782117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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