

| Catalog No. | HF996137 |
|---|---|
| Species reactivity | Human |
| Applications | FCM |
| Host species | Human |
| Isotype | IgG1, kappa |
| Clone ID | 1H4 |
| Conjugation | APC |
| Clonality | Monoclonal |
| Target | IL2-RA, IL-2R subunit alpha, TAC antigen, IL-2-RA, p55, IL2RA, IL-2 receptor subunit alpha, Interleukin-2 receptor subunit alpha, CD25 |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P01589 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Background | Interleukin-2 receptor subunit alpha (CD25/IL2RA) is a ~30 kDa protein. Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
| Note | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |

Flow-cytometry APC using anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD25 monoclonal antibody (Catalog HF996137) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.




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