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Anti-Mouse CD25/IL2RA Antibody (PC61/PC61.5.3), APC (MF996137)

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Overview
Catalog No.MF996137
Description
Anti-Mouse CD25/IL2RA Antibody (PC61/PC61.5.3), APC [PC61/PC61.5.3] (MF996137) is a rat monoclonal antibody detecting Il2ra in FCM. Suitable for Mouse.
Highlights
  • Flow Cytometry — Validated for flow cytometry applications.
  • Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivityMouse
ApplicationsFCM
Host speciesRat
IsotypeIgG1, lambda
Clone IDPC61/PC61.5.3
ConjugationAPC
Clonality Monoclonal
Target Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25, Il2ra, Il2r
Endotoxin level Please contact with the lab for this information.
Accession P01590
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Interleukin-2 receptor subunit alpha (CD25/IL2RA) is a ~30 kDa protein. Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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