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Anti-Human Fc gamma RIII/CD16 Recombinant Antibody (3G8), APC (HY386137)

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Overview
Catalog No.HY386137
Description
Anti-Human Fc gamma RIII/CD16 Recombinant Antibody (3G8), APC (HY386137) is a mouse monoclonal antibody detecting Fc gamma RIII/CD16 in ELISA, FCM, IF. Suitable for Human.
Highlights
  • ●Multi-Application — Validated across multiple applications.
  • ●APC Conjugated — Ready for direct detection without secondary antibody.
Species reactivityHuman
ApplicationsELISA, FCM, IF
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone ID3G8
ConjugationAPC
Target CD16, CD16a, CD16A, FCGR3A, Fc-gamma RIII-alpha, FcRIIIa, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A, IGFR3, CD16a antigen, Fc-gamma RIII, FCGR3, Fc-gamma RIIIa, FCG3, FcRIII, FcR-10, Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, CD16-I, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb, FcR-10, IgG Fc receptor III-1, CD16b, FCGR3B, CD16B, FCG3, FCGR3, IGFR3
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Associated products Mouse IgG1 kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Accession P08637 & O75015
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Low affinity immunoglobulin gamma Fc region receptor III-B (CD16b/FCGR3B) is a ~26 kDa protein. Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human Fc gamma RIII/CD16 Recombinant Antibody (3G8), APC

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