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Anti-Human GPC3 Antibody (SAA0134), PerCP (HW310147)

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Overview

Catalog No.	HW310147
Description	Anti-Human GPC3 Antibody (SAA0134), PerCP [SAA0134] (HW310147) is a human monoclonal antibody detecting GPC3 in ELISA, FCM, WB. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	ELISA, FCM, WB
Host species	Human
Isotype	IgG1, kappa
Clone ID	SAA0134
Conjugation	PerCP
Clonality	Monoclonal
Target	GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5
Endotoxin level	Please contact with the lab for this information.
Accession	P51654
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. 1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063 2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623 3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449 4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Datasheet

Anti-Human GPC3 Antibody (SAA0134), PerCP.pdf

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Anti-Human GPC3 Antibody (SAA0134), PerCP