Anti-Human CD340/ERBB2/HER2/NEU Antibody (A21), PE (HY286327)

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Overview

Catalog No.	HY286327
Description	Anti-Human CD340/ERBB2/HER2/NEU Antibody (A21), PE [A21] (HY286327) is a mouse monoclonal antibody detecting ERBB2 in ELISA, FCM, WB. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM, WB
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	A21
Conjugation	PE
Clonality	Monoclonal
Target	p185erbB2, HER2, NGL, Tyrosine kinase-type cell surface receptor HER2, NEU, MLN 19, Proto-oncogene Neu, MLN19, ERBB2, Proto-oncogene c-ErbB-2, CD340, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein
Endotoxin level	Please contact with the lab for this information.
Accession	P04626
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Receptor tyrosine-protein kinase erbB-2 (CD340/ERBB2) is a ~137 kDa protein. Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. CD340 is the therapeutic target of trastuzumab (Herceptin) and trastuzumab deruxtecan (Enhertu).
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using PE anti-human ERBB2 antibody. MCF-7 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ERBB2 monoclonal antibody (Catalog: HY286327, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

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Datasheet

Anti-Human CD340/ERBB2/HER2/NEU Antibody (A21), PE.pdf

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Anti-Human CD340/ERBB2/HER2/NEU Antibody (A21), PE (HY286327)

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Datasheet

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