Anti-Dog CD28 Antibody (1C6), PE (CY422127)

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Overview

Catalog No.	CY422127
Description	Anti-Dog CD28 Antibody (1C6), PE [1C6] (CY422127) is a mouse monoclonal antibody detecting CD28 in ELISA, FCM. Suitable for Dog. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Dog
Applications	ELISA, FCM
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	1C6
Conjugation	PE
Clonality	Monoclonal
Target	CD28, TP44, T-cell-specific surface glycoprotein CD28
Endotoxin level	Please contact with the lab for this information.
Accession	Q9N0N8
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	T-cell-specific surface glycoprotein CD28 is a ~25 kDa protein. Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Anti-Dog CD28 Antibody (1C6), PE.pdf

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Anti-Dog CD28 Antibody (1C6), PE (CY422127)

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