Anti-Human CEACAM-1/CD66a Recombinant Antibody (SAA0761), PE (HB880127)

Name: Anti-Human CEACAM-1/CD66a Recombinant Antibody (SAA0761), PE
Brand: abinScience
SKU: HB880127
Price: 290 USD
Availability: InStock

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Overview

Catalog No.	HB880127
Description	Anti-Human CEACAM-1/CD66a Recombinant Antibody (SAA0761), PE (HB880127) is a mouse monoclonal antibody detecting CEACAM-1/CD66a in ELISA, FCM. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●PE Conjugated — Ready for direct detection without secondary antibody.
Species reactivity	Human
Applications	ELISA, FCM
Host species	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone ID	SAA0761
Conjugation	PE
Target	CD66a, BGP-1, Biliary glycoprotein 1, CEACAM1, Carcinoembryonic antigen-related cell adhesion molecule 1, BGP, BGP1
Endotoxin level	< 10 EU/mg
Purity	>90% as determined by SDS-PAGE.
Associated products	Mouse IgG2a kappa Recombinant Isotype Control Antibody (C1.18.4), PE
Accession	P13688
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Cell adhesion molecule CEACAM1 (CD66a) is a ~57 kDa protein. Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner. Plays a role as coinhibitory receptor in immune response, insulin action and also functions as an activator during angiogenesis. Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors. Plays a role in immune response, of T cells, natural killer (NK) and neutrophils. Upon TCR/CD3 complex stimulation, inhibits TCR-mediated cytotoxicity by blocking granule exocytosis by mediating homophilic binding to adjacent cells, allowing interaction with and phosphorylation by LCK and interaction with the TCR/CD3 complex which recruits PTPN6 resulting in dephosphorylation of CD247 and ZAP70. 1. Chen, Z. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 6085-93. PMID: 18424730 2. Hosomi, S. et al. (2013) European journal of immunology 43, 2473-83. PMID: 23696226 3. Huang, YH. et al. (2015) Nature 517, 386-90. PMID: 25363763 4. Klaile, E. et al. (2005) Journal of cell science 118, 5513-24. PMID: 16291724
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using PE anti-human CD66a antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD66a monoclonal antibody (Catalog: HB880127, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet