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Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC (HY257617)

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Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC

Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC

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Datasheet

Overview

Catalog No.	HY257617
Description	Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC [SAA2209] (HY257617) is a human monoclonal antibody detecting MS4A1 in ELISA, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	ELISA, FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	SAA2209
Conjugation	FITC
Clonality	Monoclonal
Target	B-lymphocyte surface antigen B1, Membrane-spanning 4-domains subfamily A member 1, MS4A1, Leukocyte surface antigen Leu-16, B-lymphocyte antigen CD20, Bp35, CD20
Endotoxin level	Please contact with the lab for this information.
Purification	Affinity chromatography.
Accession	P11836
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	B-lymphocyte antigen CD20 (MS4A1) is a ~33 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva). 1. Li, H. et al. (2003) The Journal of biological chemistry 278, 42427-34. PMID: 12920111 2. Tedder, TF. et al. (1985) Journal of immunology (Baltimore, Md. : 1950) 135, 973-9. PMID: 3925015 3. Bubien, JK. et al. (1993) The Journal of cell biology 121, 1121-32. PMID: 7684739 4. Polyak, MJ. et al. (2008) The Journal of biological chemistry 283, 18545-52. PMID: 18474602
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow Cytometry

Flow-cytometry using FITC anti-Human CD20 antibody. Daudi cells were stained with an irrelevant antibody (Red Histogram) or a FITC anti-Human CD20 antibody monoclonal antibody (Catalog # HY257617 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC.pdf

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Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC