



| Catalog No. | HB102407 |
|---|---|
| Species reactivity | Human |
| Applications | ELISA, FCM |
| Host species | Human |
| Isotype | IgG1, kappa |
| Clone ID | SAA2216 |
| Clonality | Monoclonal |
| Target | PEMT, CA 15-3, H23AG, Peanut-reactive urinary mucin, PEM, EMA, Cancer antigen 15-3, MUC-1, Episialin, MUC1-CT, MUC1-beta, Tumor-associated mucin, CD227, Polymorphic epithelial mucin, KL-6, MUC1-alpha, MUC1, PUM, Krebs von den Lungen-6, MUC1-NT, Tumor-associated epithelial membrane antigen, Mucin-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P15941 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |

Flow-cytometry using anti-human CD227 antibody. MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD227 antibody monoclonal antibody (Catalog # HB102407 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD227 antibody. CD227 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD227 antibody monoclonal antibody (Catalog # HB102407 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.




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