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GMP Grade Human IL12 (Tag Free) (HV282021)

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Overview
Catalog No.HV282021
Expression systemMammalian cells
AccessionP29460(IL12 p40), P29459(IL12 p35)
Protein lengthIle23-Ser328&Arg23-Ser219
ApplicationsELISA, Functional assay
SpeciesHomo sapiens (Human)
Biological activity Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is <0.5 ng/mL.
Nature Recombinant
Endotoxin level <0.01 EU/ug
Purity >95% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% Mannitol, 0.01% Tween 80, pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, IL12B, Interleukin-12 subunit beta, NK cell stimulatory factor chain 2, NKSF2, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, IL12A, Interleukin-12 subunit alpha, NK cell stimulatory factor chain 1, NKSF1
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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