Human TIM-3 Antibody Pair Set (HV029908)

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Overview

Catalog No.	HV029908
Description	Human TIM-3 Antibody Pair Set (HV029908) is a capture: human; detection: rabit antibody detecting TIM-3 in ELISA. Suitable for Human. Highlights ●Detection: Biotin Conjugated — Ready for direct detection without secondary antibody.
Sensitivity	5.98 pg/mL
Range	31.3-2,000 pg/mL
Species reactivity	Human
Host species	Capture: Human, Detection: Rabbit
Immunogen	TIM-3
Conjugation	Detection: Biotin
Tested applications	Sandwich ELISA
Target	CD366, HAVCR2, HAVcr-2, Hepatitis A virus cellular receptor 2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIM3, TIMD-3, TIMD3
Specificity	TIM-3
Accession	Q8TDQ0
Applications	ELISA
Form	Liquid
Storage buffer	Capture: 0.01M PBS pH 7.4; Detection: 0.01M PBS pH 7.4, 50% glycerol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Detection method	Detection method
Assay type	Sandwich ELISA
Specifications	Reagents are sufficient for at least 1 x 96 well plates using recommended protocol.
Background	Hepatitis A virus cellular receptor 2 (CD366/TIM-3) is a ~33 kDa protein. Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. 1. Gorman, JV. et al. (2014) Immunologic research 59, 56-65. PMID: 24825777 2. Monney, L. et al. (2002) Nature 415, 536-41. PMID: 11823861 3. Sánchez-Fueyo, A. et al. (2003) Nature immunology 4, 1093-101. PMID: 14556005 4. Clayton, KL. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 782-91. PMID: 24337741 5. Tomkowicz, B. et al. (2015) PloS one 10, e0140694. PMID: 26492563 6. Gautron, AS. et al. (2014) European journal of immunology 44, 2703-2711. PMID: 24838857 7. Zhu, C. et al. (2005) Nature immunology 6, 1245-52. PMID: 16286920 8. Leitner, J. et al. (2013) PLoS pathogens 9, e1003253. PMID: 23555261 9. Gleason, MK. et al. (2012) Blood 119, 3064-72. PMID: 22323453
Note	For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Images

Standard Curve

Standard Curve of Human TIM-3 Antibody Pair Set

References

Datasheet

Human TIM-3 Antibody Pair Set.pdf

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Protocol

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

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Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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