cAMP ELISA KIT (YP051018)

Overview

Catalog No.	YP051018
Description	cAMP ELISA KIT (YP051018) for research applications.
Sample type	Plasma, Serum
Sensitivity	1.56 pmol/mL
Range	1.56-200 pmol/mL
Applications	ELISA
Detection method	Colorimetric
Assay type	Quantitative
Recovery	80-120%
Shipping	2-8 °C
Stability and Storage	The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.
Specifications	cAMP
Background	Adenosine 3', 5'-cyclic monophosphate (cAMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors(GPCRs). Bioyeargene cAMP ELISA Kit is based on the competition between HRP-labeled cAMP and free cAMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cAMP MAb) binding sites. Because the concentration of the HRP-labeled cAMP is held constant while the concentration of cAMP varies, the amount of HRP-labeled cAMP that is able to bind to the cAMP MAb will be inversely proportional to the concentration of cAMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50(50% B/B0) of approximately 12 pmol/mL and a detection limit of approximately 0.15 pmol/mL.
Note	For research use only.

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References

Datasheet

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Overview