BDBV Nucleoprotein Human IgG ELISA Kit (VK059018)

Overview

Catalog No.	VK059018
Description	BDBV Nucleoprotein Human IgG ELISA Kit (VK059018) — Quantitative sandwich ELISA for Nucleoprotein.
Sample type	Serum and plasma
Sensitivity	0.85 ng/mL
Range	3.1 - 200 ng/mL
Species reactivity	Human anti-BDBV nucleoprotein IgG
Target	Bundibugyo ebolavirus nucleoprotein
Accession	R4QJ68
Applications	ELISA
Detection method	Colorimetric
Assay type	Indirect
Precision	Intra-CV and Inter-CV < 15%
Recovery	80-120%
Shipping	2-8 ℃
Stability and Storage	The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.
Background	Nucleoprotein (NP) is a ~56 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.
Note	For research use only

Images

References

Datasheet

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Overview