Catalog No. | HF564016 |
---|---|
Species reactivity | Human |
Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
Host species | Human |
Isotype | IgG2-kappa |
Expression system | Mammalian Cells |
Clonality | Monoclonal |
Target | B7RP1, B7-related protein 1, CD275, ICOSL, ICOSLG, KIAA0653, B7 homolog 2, ICOS ligand, B7RP-1, B7H2, B7-H2, B7-like protein Gl50 |
Endotoxin level | Please contact the lab for this information. |
Purity | >95% purity as determined by SDS-PAGE. |
Purification | Protein A/G purified from cell culture supernatant. |
Accession | O75144 |
Form | Liquid |
Storage buffer | 0.01M PBS, pH 7.4. |
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. |
Alternate Names | AMG557, MEDI-5872, 1523164-68-2 |
Background | Prezalumab (AMG 557) is a monoclonal antibody targeting ICOSL that has synergistic anti-inflammatory activity with CD28 antibody inhibitors. |
Note | For research use only. Not suitable for clinical or therapeutic use. |
SEC-HPLC detection for Research Grade Prezalumab.
Detects CD275/ICOSLG in indirect ELISAs.
SDS-PAGE for Research Grade Prezalumab.
Flow-cytometry using anti-human CD275 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD275 monoclonal antibody (Catalog: HF564016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
+33(0)3 90 20 54 70
19 rue de la Haye 67300 Schiltigheim France