Research Grade Abatacept (HW630296)

Name: Research Grade Abatacept
Brand: abinScience
SKU: HW630296
Price: 220 USD
Availability: InStock

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Overview

Catalog No.	HW630296
Description	Abatacept (HW630296) is a research-grade recombinant antibody targeting CD80. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-Fc
Expression system	Mammalian cells
Clonality	Monoclonal
Tested applications	FCM
Target	CD80, BB1, T-lymphocyte activation antigen CD80, CD28LG, B7, CD28LG1, LAB7, Activation B7-1 antigen, CTLA-4 counter-receptor B7.1, BU63, B70, CD28LG2, CTLA-4 counter-receptor B7.2, Activation B7-2 antigen, T-lymphocyte activation antigen CD86, CD86, FUN-1
Endotoxin level	< 10 EU/mg
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P16410, CAS: 332348-12-6
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Cytotoxic T-lymphocyte protein 4 (CD80/B7-1 & CD86) is a ~24 kDa protein. Inhibitory receptor acting as a major negative regulator of T-cell responses. Acts as a decoy receptor: the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. 1. Schwartz, JC. et al. (2001) Nature 410, 604-8. PMID: 11279501 2. Stamper, CC. et al. (2001) Nature 410, 608-11. PMID: 11279502 3. Teft, WA. et al. (2006) Annual review of immunology 24, 65-97. PMID: 16551244 4. Linsley, PS. et al. (1991) The Journal of experimental medicine 174, 561-9. PMID: 1714933 5. Zheng, Y. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 1683-91. PMID: 18641304 6. Ramagopal, UA. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E4223-E4232. PMID: 28484017
Note	For research use only. Not suitable for clinical or therapeutic use.

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Bioactivity

SEC-HPLC detection for Research Grade Abatacept.
Flow CytoMetry

Flow-cytometry using APC anti-human CD80 &/or CD86 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD80 &/or CD86 monoclonal antibody (Catalog: HW630296, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD80 &/or CD86 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD80 &/or CD86 monoclonal antibody (Catalog: HW630296, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Research Grade Abatacept
Bioactivity

Detected by Anti-Abatacept antibody SAA2444 (Catalog No: AW630013) in indirect ELISA

References

Datasheet

Research Grade Abatacept.pdf

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Formula

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Research Grade Abatacept (HW630296)

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