Anti-Human PD-L1/B7-H1 Reference Antibody (Atezolizumab, RUO) (HV974016)

Name: Anti-Human PD-L1/B7-H1 Reference Antibody (Atezolizumab, RUO)
Brand: abinScience
SKU: HV974016
Price: 176 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.	HV974016
Description	_x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Atezolizumab (HV974016) is a research-grade recombinant antibody targeting CD1. Produced in mammalian cells with native-like glycosylation. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Highlights _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ ●Research Grade — For PK/PD studies, assay development, and ADA research. _x005F_x000D_ ●Native Glycosylation — Mammalian expression ensures native-like patterns. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Resources _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ ●Flow Cytometry Protocol for PD-L1 (CD274) Detection — Validated protocol on protocols.io _x005F_x000D_ _x005F_x000D_ _x005F_x000D_
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Expression system	Mammalian cells
Clonality	Monoclonal
Target	B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9NZQ7
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	MPDL3280A, RG7446, 1380723-44-3
Background	Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~33 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi). _x005F_x000D_ _x005F_x000D_ 1. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 _x005F_x000D_ _x005F_x000D_ 2. Mezzadra, R. et al. (2017) Nature 549, 106-110. PMID: 28813410 _x005F_x000D_ _x005F_x000D_ 3. Burr, ML. et al. (2017) Nature 549, 101-105. PMID: 28813417 _x005F_x000D_ _x005F_x000D_ 4. Huang, X. et al. (2019) Cancer immunology research 7, 1580-1590. PMID: 31399419 _x005F_x000D_ _x005F_x000D_ 5. Kennedy, A. et al. (2023) The EMBO journal 42, e111556. PMID: 36727298 _x005F_x000D_ _x005F_x000D_ 6. Dong, H. et al. (1999) Nature medicine 5, 1365-9. PMID: 10581077 _x005F_x000D_ _x005F_x000D_ 7. Hou, J. et al. (2020) Nature cell biology 22, 1264-1275. PMID: 32929201 _x005F_x000D_
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SEC-HPLC

SEC-HPLC detection for Research Grade Atezolizumab.
Bioactivity

Detects CD274/PD-L1/B7-H1 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Atezolizumab
Flow Cytometry

Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog HV974016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD274 monoclonal antibody (Catalog: HV974016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.