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Research Grade Anti-SARS-CoV-2 S2 Protein (CV3-25) (VK749026)

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Research Grade Anti-SARS-CoV-2 S2 Protein (CV3-25)

Research Grade Anti-SARS-CoV-2 S2 Protein (CV3-25)

Research Grade Anti-SARS-CoV-2 S2 Protein (CV3-25)

Research Grade Anti-SARS-CoV-2 S2 Protein (CV3-25)

Research Grade Anti-SARS-CoV-2 S2 Protein (CV3-25)

Research Grade Anti-SARS-CoV-2 S2 Protein (CV3-25)

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Spec:

100ug
1mg

Number:

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Overview
Images
References
Datasheet

Overview

Catalog No.	VK749026
Description	Anti-SARS-CoV-2 S2 Protein (CV3-25) (VK749026) is a research-grade recombinant antibody targeting Spike protein S2'. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	SARS-CoV-2(2019-nCoV)
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1
Clone ID	CV3-25
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Spike protein S1, Spike protein S2, Spike protein S2', S, 2
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P0DTC2
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Background	Spike glycoprotein (Spike glycoprotein 2) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors. 1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651 2. Walls, AC. et al. (2020) Cell 181, 281-292.e6. PMID: 32155444 3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 33607086 4. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 34561887 5. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 32075877 6. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 32221306 7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715 8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763 9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294 10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-SARS-CoV-2 S2 Protein (CV3-25).
Western blot

Western blot analysis was performed using anti-SARS-CoV-2 S2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant SARS-CoV-2 S2/Spike S2 (ECD) (Catalog No: VK749032)
Lane 2: negative control
Bioactivity

Detects recombinant SARS-CoV-2 S2/Spike S2 (ECD) (Catalog No: VK749032) in indirect ELISA

References

Datasheet

Research Grade Anti-SARS-CoV-2 S2 Protein (CV3-25).pdf

Formula

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Research Grade Anti-SARS-CoV-2 S2 Protein (CV3-25)