Anti-Human ErbB2/Her2 Reference Antibody (MEDI4276, RUO) (HY286566)

Name: Anti-Human ErbB2/Her2 Reference Antibody (MEDI4276, RUO)
Brand: abinScience
SKU: HY286566
Price: 176 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.	HY286566
Species reactivity	Human
Applications	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Host species	Human
Clonality	Monoclonal
Isotype	scFv-Human IgG1, kappa
Clone ID	MEDI4276
Tested applications	WB, FCM
Target	CD340, EC:2.7.10.1, ERBB2, HER2, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEU, NGL, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2
Endotoxin level	< 10 EU/mg
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P04626
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	MEDI4276
Background	Receptor tyrosine-protein kinase erbB-2 (CD340/ERBB2) is a ~137 kDa protein. Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. CD340 is the therapeutic target of trastuzumab (Herceptin) and trastuzumab deruxtecan (Enhertu).
References	1. Pegram MD, et al. First-in-Human, Phase 1 Dose-Escalation Study of Biparatopic Anti-HER2 Antibody-Drug Conjugate MEDI4276 in Patients with HER2-positive Advanced Breast or Gastric Cancer. Mol Cancer Ther. 2021 Aug;20(8):1442-1453. [HY286566]
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

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SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD340/ERBB2/HER2/NEU (MEDI4276)
Flow cytometry

Flow-cytometry using anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 monoclonal antibody (Catalog HY286566, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects recombinant CD340 (Catalog No: HY286011) in indirect ELISA.
Western blot

Lane 1: recombinant human CD340/ERBB2 (Catalog No: HY286011)
Lane 2: 1*loading buffer

References

Datasheet

Anti-Human ErbB2/Her2 Reference Antibody (MEDI4276, RUO).pdf

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Recombinant Proteins

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Anti-Human ErbB2/Her2 Reference Antibody (MEDI4276, RUO) (HY286566)

Overview

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References

Datasheet

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