Research Grade Gevokizumab (HF943026)

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Spec:

100ug
1mg

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Overview
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References
Datasheet

Overview

Catalog No.	HF943026
Description	Research Grade Gevokizumab (HF943026) is a humanized monoclonal antibody detecting Interleukin-1 beta in ELISA, Bioactivity: FACS, Functional assay, Research in vivo. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Humanized
Isotype	IgG2-kappa
Expression system	Mammalian Cells
Species	Human
Clonality	Monoclonal
Target	Interleukin-1 beta, IL1B, Catabolin, IL1F2, IL-1 beta
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01584
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternate Names	XOMA 052, 1129435-60-4
Background	Interleukin-1 beta (Interleukin-1 beta,IL1B,Catabolin,IL1F2,IL-1 beta) is a ~30 kDa protein. Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. 1. Tominaga, K. et al. (2000) International immunology 12, 151-60. PMID: 10653850 2. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819 3. LaRock, CN. et al. (2016) Science immunology 1. PMID: 28331908 4. Van Damme, J. et al. (1985) Nature 314, 266-8. PMID: 3920526 5. Tian, XX. et al. (2021) International endodontic journal 54, 916-925. PMID: 33377178 6. Xia, S. et al. (2021) Nature 593, 607-611. PMID: 33883744
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SEC-HPLC

SEC-HPLC detection for Research Grade Gevokizumab.
Bioactivity

Detects Human IL1B/IL1F2 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Gevokizumab.
Western blot

Western blot analysis was performed using anti-IL1B/IL1F2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL1B/IL1F2 (Catalog No: HF943012), 50ng
Lane 2: recombinant human IL1B/IL1F2 (Catalog No: HF943012), 30ng
Lane 3: recombinant human IL1B/IL1F2 (Catalog No: HF943012), 20ng
Lane 4: recombinant human IL1B/IL1F2 (Catalog No: HF943012), 10ng