Recombinant Human CD134/TNFRSF4/OX40 Protein, C-His (HW342011)

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Datasheet

Overview

Catalog No.	HW342011
Description	Recombinant Human CD134/TNFRSF4/OX40 Protein, C-His (HW342011) expressed in Mammalian Cells, spanning Met1-Ala216. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P43489
Protein length	Met1-Ala216
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.88 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ACT35 antigen, TXGP1L, TAX transcriptionally-activated glycoprotein 1 receptor, Tumor necrosis factor receptor superfamily member 4, TNFRSF4, OX40L receptor, CD134
Background	Tumor necrosis factor receptor superfamily member 4 (CD134/OX40) is a ~29 kDa protein. Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CD134/TNFRSF4/OX40 Protein, C-His
Bioactivity

Detects Ivuxolimab in indirect ELISAs.
Bioactivity

Detects Rocatinlimab in indirect ELISAs.
Bioactivity

Detects Tavolimab in indirect ELISAs.
Bioactivity

Detects Telazorlimab in indirect ELISAs.
Western blot

Detected by Anti-CD134/TNFRSF4/OX40 monoclonal antibody (Catalog No: HV612036)
Western blot

Detected by Anti-CD134/TNFRSF4/OX40 monoclonal antibody (Catalog No: HW342166)
Bioactivity

Detected by Research Grade Anti-Human CD134/TNFRSF4/OX40 (BMS 986178) (Catalog No: HW342106) in indirect ELISA
Bioactivity

Detected by Research Grade Anti-Human CD134/TNFRSF4/OX40 (GSK 3174998) (Catalog No: HW342146) in indirect ELISA
Bioactivity

Detected by Gimistotug (Catalog No: HW342196) in indirect ELISA.