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Recombinant Human CD16b/FCGR3B Protein, C-His (HF886011)

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Recombinant Human CD16b/FCGR3B Protein, C-His

Recombinant Human CD16b/FCGR3B Protein, C-His

Recombinant Human CD16b/FCGR3B Protein, C-His

Recombinant Human CD16b/FCGR3B Protein, C-His

Recombinant Human CD16b/FCGR3B Protein, C-His

Recombinant Human CD16b/FCGR3B Protein, C-His

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Overview
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References
Datasheet

Overview

Catalog No.	HF886011
Description	Recombinant Human CD16b/FCGR3B Protein, C-His (HF886011) expressed in Mammalian Cells, spanning Met1-Ser200. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	O75015
Protein length	Met1-Ser200
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	23.56 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	IGFR3, FCGR3B, CD16b, Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII, IgG Fc receptor III-1, FCGR3, CD16B, Fc-gamma RIII-beta, Fc-gamma RIIIb, FCG3, FcRIII, FcR-10, FcRIIIb
Background	Low affinity immunoglobulin gamma Fc region receptor III-B (CD16b/FCGR3B) is a ~26 kDa protein. Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CD16b/FCGR3B Protein, C-His
Bioactivity

Detected by SAA2149 (Catalog No: HY386023) in indirect ELISA.
Bioactivity

Detected by GMA-161 (Catalog No: HY386016) in indirect ELISA.

References

HF886011-SDS-PAGE-1.jpg

Datasheet

Recombinant Human CD16b/FCGR3B Protein, C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

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Stock Solution

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Working Solution

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Recombinant Human CD16b/FCGR3B Protein, C-His