Home > Products > Recombinant Proteins > Immune Checkpoint Proteins

Recombinant Sindbis virus(SINV) Spike glycoprotein E1, C-His (VK002041)

Datasheet Share product Copy info

Recombinant Sindbis virus(SINV) Spike glycoprotein E1, C-His

Recombinant Sindbis virus(SINV) Spike glycoprotein E1, C-His

Price(USD): $

Spec:

50ug
100ug
1mg

Number:

Contact us

Overview
Images
References
Datasheet

Overview

Catalog No.	VK002041
Description	Recombinant Sindbis virus(SINV) Spike glycoprotein E1, C-His (VK002041) expressed in Mammalian Cells, spanning Tyr807-Ser1214. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P03316
Protein length	Tyr807-Ser1214
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Sindbis virus (SINV)
Nature	Recombinant
Target	glycoprotein E1
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	46.88 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Structural polyprotein (glycoprotein E1) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions. 1. Zhang, W. et al. (2002) Journal of virology 76, 11645-58. PMID: 12388725 2. Mukhopadhyay, S. et al. (2006) Structure (London, England : 1993) 14, 63-73. PMID: 16407066 3. Tang, J. et al. (2011) Journal of molecular biology 414, 442-59. PMID: 22001018 4. Paredes, AM. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9095-9. PMID: 8415660 5. Choi, HK. et al. (1991) Nature 354, 37-43. PMID: 1944569 6. Owen, KE. et al. (1997) Virology 230, 187-96. PMID: 9143274 7. DeTulleo, L. et al. (1998) The EMBO journal 17, 4585-93. PMID: 9707418 8. Coombs, K. et al. (1987) Journal of molecular biology 195, 359-71. PMID: 3656418 9. Landers, VD. et al. (2021) Viruses 13. PMID: 33673546
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Sindbis virus(SINV) Spike glycoprotein E1, C-His

References

Datasheet

Recombinant Sindbis virus(SINV) Spike glycoprotein E1, C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

=

Concentration

×

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

×

V₁ (Stock Vol.)

=

Working Solution

C₂ (Working Conc.)

×

V₂ (Working Vol.)

Recommendation

Contact us for custom quotes, bulk requests and any other issues.

Mail: support@abinScience.com

Distributor list

Recombinant Sindbis virus(SINV) Spike glycoprotein E1, C-His