Human OAS2 Recombinant Protein (N-His) (HB125012)

Name: Human OAS2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB125012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB125012
Description	Recombinant Human OAS2 Protein, N-His (HB125012) expressed in E. coli, spanning Val11-His346. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P29728
Protein length	Val11-His346
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	41.19 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	p69 OAS / p71 OAS, OAS2, 2-5A synthase 2, p69OAS / p71OAS, 2'-5'-oligoadenylate synthase 2, (2-5')oligo(A) synthase 2
Background	2'-5'-oligoadenylate synthase 2 (OAS2) is a ~82 kDa protein. Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. Activated by detection of double stranded RNA (dsRNA): polymerizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNASEL) leading to its dimerization and subsequent activation. Activation of RNASEL leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNASEL-dependent pathway or an alternative antiviral pathway independent of RNASEL. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. 1. Sarkar, SN. et al. (1999) The Journal of biological chemistry 274, 25535-42. PMID: 10464285 2. Sarkar, SN. et al. (1999) The Journal of biological chemistry 274, 1848-55. PMID: 9880569 3. Hartmann, R. et al. (2001) FEBS letters 507, 54-8. PMID: 11682059 4. Kristiansen, H. et al. (2011) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 31, 41-7. PMID: 21142819
Note	For research use only.

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SDS-PAGE for Recombinant Human OAS2 Protein, N-His

References

HB125012-SDS-PAGE-1.jpg

Datasheet

Human OAS2 Recombinant Protein (N-His).pdf

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Human OAS2 Recombinant Protein (N-His) (HB125012)

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