Human S100B Recombinant Protein (N-His) (HY256012)

Name: Human S100B Recombinant Protein (N-His)
Brand: abinScience
SKU: HY256012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY256012
Description	Recombinant Human S100B Protein, N-His (HY256012) expressed in E. coli, spanning Met1-Glu92. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P04271
Protein length	Met1-Glu92
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	11.81 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	S100 calcium-binding protein B, S100B, S-100 protein subunit beta, Protein S100-B, S-100 protein beta chain
Background	Protein S100-B (S100B) is a ~10 kDa protein. Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain. Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation. Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue. 1. Ostendorp, T. et al. (2011) Biochimica et biophysica acta 1813, 1083-91. PMID: 20950652 2. Baudier, J. et al. (1984) Biochimica et biophysica acta 790, 164-73. PMID: 6487634 3. Gilquin, B. et al. (2010) Molecular and cellular biology 30, 2724-36. PMID: 20351179 4. Yamaguchi, F. et al. (2012) The Journal of biological chemistry 287, 13787-98. PMID: 22399290
Note	For research use only.

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SDS-PAGE for Recombinant Human S100B Protein, N-His

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Datasheet

Human S100B Recombinant Protein (N-His).pdf

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Human S100B Recombinant Protein (N-His) (HY256012)

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