Human OGDH Recombinant Protein (N-His) (HX199012)

Name: Human OGDH Recombinant Protein (N-His)
Brand: abinScience
SKU: HX199012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX199012
Description	Recombinant Human OGDH Protein, N-His (HX199012) expressed in E. coli, spanning Ala256-Lys582. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q02218
Protein length	Ala256-Lys582
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	39.68 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Alpha-ketoglutarate dehydrogenase, OGDC-E1, OGDH, 2-oxoglutarate dehydrogenase complex component E1, 2-oxoglutarate dehydrogenase, mitochondrial
Background	2-oxoglutarate dehydrogenase complex component E1 (OGDH) is a ~115 kDa protein. 2-oxoglutarate dehydrogenase (E1o) component of the 2-oxoglutarate dehydrogenase complex (OGDHC). Participates in the first step, rate limiting for the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) catalyzed by the whole OGDHC. Catalyzes the irreversible decarboxylation of 2-oxoglutarate (alpha-ketoglutarate) via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine-residue succinyltransferase or DLST). Plays a key role in the Krebs (citric acid) cycle, which is a common pathway for oxidation of fuel molecules, including carbohydrates, fatty acids, and amino acids. Can catalyze the decarboxylation of 2-oxoadipate in vitro, but at a much lower rate than 2-oxoglutarate. 1. Armstrong, CT. et al. (2014) The Biochemical journal 459, 369-81. PMID: 24495017 2. Nemeria, NS. et al. (2014) The Journal of biological chemistry 289, 29859-73. PMID: 25210035 3. Nemeria, NS. et al. (2017) Free radical biology & medicine 108, 644-654. PMID: 28435050 4. Zhong, Y. et al. (2022) Biochemical and biophysical research communications 602, 120-126. PMID: 35272141 5. Wang, Y. et al. (2017) Nature 552, 273-277. PMID: 29211711
Note	For research use only.

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SDS-PAGE for Recombinant Human OGDH Protein, N-His

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Datasheet

Human OGDH Recombinant Protein (N-His).pdf

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Human OGDH Recombinant Protein (N-His) (HX199012)

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