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Human CENPE Recombinant Protein (N-His) (HX024012)

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Human CENPE Recombinant Protein (N-His)
Human CENPE Recombinant Protein (N-His)
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Overview
Catalog No.HX024012
Description
Recombinant Human CENPE Protein, N-His (HX024012) expressed in E. coli, spanning Ala2450-Arg2590. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ02224
Protein lengthAla2450-Arg2590
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 18.64 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCENP-E, CENPE, Kinesin-related protein CENPE, Centromere-associated protein E, Centromere protein E, Kinesin-7
Background

Centromere-associated protein E (CENPE) is a ~316 kDa protein. Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated. Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends. Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized.

1. Shrestha, RL. et al. (2013) Current biology : CB 23, 1514-26. PMID: 23891108
2. Bancroft, J. et al. (2015) Journal of cell science 128, 171-84. PMID: 25395579
3. Thrower, DA. et al. (1995) The EMBO journal 14, 918-26. PMID: 7889940
4. Barisic, M. et al. (2015) Science (New York, N.Y.) 348, 799-803. PMID: 25908662
5. Gudimchuk, N. et al. (2013) Nature cell biology 15, 1079-1088. PMID: 23955301
6. Iemura, K. et al. (2015) Nature communications 6, 6447. PMID: 25743205
7. Liu, D. et al. (2007) The Journal of biological chemistry 282, 21415-24. PMID: 17535814
Note For research use only.
Images
  • Human CENPE Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human CENPE Protein, N-His

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Human CENPE Recombinant Protein (N-His)

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