ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
29.08 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
MAP3K7IP1, TGF-beta-activated kinase 1-binding protein 1, TAK1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, TAB1
Background
TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 (TAB1) is a ~54 kDa protein. Key adapter protein that plays an essential role in JNK and NF-kappa-B activation and proinflammatory cytokines production in response to stimulation with TLRs and cytokines. Mechanistically, associates with the catalytic domain of MAP3K7/TAK1 to trigger MAP3K7/TAK1 autophosphorylation leading to its full activation. Similarly, associates with MAPK14 and triggers its autophosphorylation and subsequent activation. In turn, MAPK14 phosphorylates TAB1 and inhibits MAP3K7/TAK1 activation in a feedback control mechanism. Also plays a role in recruiting MAPK14 to the TAK1 complex for the phosphorylation of the TAB2 and TAB3 regulatory subunits.
1. Pathak, S. et al. (2012) The EMBO journal 31, 1394-404. PMID: 22307082
2. Yang, CY. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 1547-57. PMID: 24403530
3. Sakurai, H. et al. (2000) FEBS letters 474, 141-5. PMID: 10838074
4. Charlaftis, N. et al. (2014) The EMBO journal 33, 2581-96. PMID: 25260751
5. Ma, M. et al. (2023) Molecular cell 83, 3885-3903.e5. PMID: 37832545
6. Ge, B. et al. (2002) Science (New York, N.Y.) 295, 1291-4. PMID: 11847341
7. Thapa, D. et al. (2018) Molecular and cellular biology 38. PMID: 29229647
8. Cheung, PC. et al. (2003) The EMBO journal 22, 5793-805. PMID: 14592977
9. Mendoza, H. et al. (2008) The Biochemical journal 409, 711-22. PMID: 18021073
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human TAB1 Protein, N-His
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