Human MTARC1 Recombinant Protein (N-His) (HV197012)

Name: Human MTARC1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV197012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV197012
Description	Recombinant Human MTARC1 Protein, N-His (HV197012) expressed in E. coli, spanning Arg41-Leu335. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q5VT66
Protein length	Arg41-Leu335
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	35.74 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	MTARC1, Molybdenum cofactor sulfurase C-terminal domain-containing protein 1, Mitochondrial amidoxime-reducing component 1, MARC1, mARC1, Moco sulfurase C-terminal domain-containing protein 1, MOSC1, MOSC domain-containing protein 1
Background	Mitochondrial amidoxime-reducing component 1 (MTARC1) is a ~37 kDa protein. Catalyzes the reduction of N-oxygenated molecules, acting as a counterpart of cytochrome P450 and flavin-containing monooxygenases in metabolic cycles. As a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability. May be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenous nitric oxide levels and biosynthesis. Postulated to cleave the N-OH bond of N-hydroxylated substrates in concert with electron transfer from NADH to cytochrome b5 reductase then to cytochrome b5, the ultimate electron donor that primes the active site for substrate reduction. 1. Gruenewald, S. et al. (2008) Journal of medicinal chemistry 51, 8173-7. PMID: 19053771 2. Kotthaus, J. et al. (2011) The Biochemical journal 433, 383-91. PMID: 21029045 3. Kubitza, C. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 11958-11963. PMID: 30397129
Note	For research use only.

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SDS-PAGE for Recombinant Human MTARC1 Protein, N-His

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Datasheet

Human MTARC1 Recombinant Protein (N-His).pdf

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Human MTARC1 Recombinant Protein (N-His) (HV197012)

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