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Recombinant MERS-CoV S/Spike glycoprotein Protein, C-His (VK800011)

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Recombinant MERS-CoV S/Spike glycoprotein Protein, C-His

Recombinant MERS-CoV S/Spike glycoprotein Protein, C-His

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Overview
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Datasheet

Overview

Catalog No.	VK800011
Description	Recombinant MERS-CoV S/Spike glycoprotein Protein, C-His (VK800011) expressed in Mammalian Cells. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	K9N5Q8
Protein length	Glu367-Asn 602
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Middle East respiratory syndrome-related coronavirus (MERS-CoV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	30.72 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Spike protein S1, MERS-CoV
Background	Spike glycoprotein is a ~149 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Interacts with host DPP4 to mediate virla entry.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant MERS-CoV S/Spike glycoprotein Protein, C-His

References

VK800011-SDS-PAGE-1.jpg

Datasheet

Recombinant MERS-CoV S/Spike glycoprotein Protein, C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

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Concentration

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Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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V₂ (Working Vol.)

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Recombinant MERS-CoV S/Spike glycoprotein Protein, C-His