Human KDM6B Recombinant Protein (N-His) (HT282012)

Name: Human KDM6B Recombinant Protein (N-His)
Brand: abinScience
SKU: HT282012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT282012
Description	Recombinant Human KDM6B Protein, N-His (HT282012) expressed in E. coli, spanning Pro1186-Arg1643. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O15054
Protein length	Pro1186-Arg1643
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	54.23 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	JMJD3, [histone H3]-trimethyl-L-lysine(27) demethylase 6B, Jumonji domain-containing protein 3, KDM6B, JmjC domain-containing protein 3, KIAA0346, Lysine demethylase 6B, Lysine-specific demethylase 6B
Background	Lysine-specific demethylase 6B (KDM6B) is a ~176 kDa protein. Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex. 1. Agger, K. et al. (2007) Nature 449, 731-4. PMID: 17713478 2. De Santa, F. et al. (2007) Cell 130, 1083-94. PMID: 17825402 3. Lan, F. et al. (2007) Nature 449, 689-94. PMID: 17851529 4. Hong, S. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 18439-44. PMID: 18003914
Note	For research use only.